In article <CDH92t.1zL at umdnj.edu>, gold at umdnj.edu (Bert Gold) writes:
|> OK, so I am new to GCG land.
|>|> I've been using Pustell's programs, DNAstar, EUGENE and various and
|> other sundry packages.
|>|> But, I started using GCG last week, and I like it!
|>|> (When have you last heard a certified gene jock say he liked anything?)
|>|> At any rate, they hired me to do a bunch of PCR.
|>|> So I'm asking, Does GCG have a subroutine designed to check or pick,
|> good PCR primers for a given sequence?
|>|> I eagerly await your kind replies.
|>|> Bert Gold, Ph.D.
|> Temple University, Philadelphia
|>|> currentl addresses: gold at rwja.umdnj.edu|>gold at soma.umdnj.edu|>bgold at hoh.mbl.edu|>gold at astro.ocis.temple.edu|>bert at sgi1.fels.temple.edu
The answer is that one has been promised and is forthcoming at some future date.
In the meantime there is a shell program base on the GCG FOLD program that I have
devised for analyzing oligonucleotide interactions to help optomize primer pairs.
This was published in BioTechniques 13: 914-917 (1992). I would be happy to
e-mail anyone a copy of the program and some notes which I have compiled about
other programs which I have found useful in addition to the shell program.
Sincerely,
Norman L. Eberhardt, Ph.D.
Associate Professor
Departments of Medicine and
Biochemistry/Molecular Biology
4-407 Alfred
Mayo Clinic
Rochester, MN 55905
eberhardt at mayo.edu
