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[Glycosci] Querry regarding silver staining for glycoproteins

D.Lefeber from neuro.umcn.nl via glycosci%40net.bio.net (by D.Lefeber from neuro.umcn.nl)
Mon Oct 26 06:40:25 EST 2009

Dear all,

Does someone know an interested researcher for a post-doc on glycan mass
spectrometry, as atteched?

Kind regards,


Please note address change!
Dr. D.J. Lefeber, PhD
Clinical Biochemical Geneticist
Glycosylation & Lysosomal disorders
Department of Laboratory Medicine
830 - Laboratory of Genetic Endocrine and Metabolic Diseases
Radboud University Nijmegen Medical Centre 
Geert Grooteplein 10
6525 GA Nijmegen
The Netherlands
D.Lefeber from neuro.umcn.nl
phone: +31 24 3614428 / 3953
fax:   +31 24 3618900

-----Oorspronkelijk bericht-----
Van: glycosci-bounces from oat.bio.indiana.edu
[mailto:glycosci-bounces from oat.bio.indiana.edu] Namens Mr.Pradeep Kumar
Iyer Group Leader, Bio Tech, HRF
Verzonden: dinsdag 20 oktober 2009 6:26
Aan: glycosci from magpie.bio.indiana.edu; methods from magpie.bio.indiana.edu
Onderwerp: [Glycosci] Querry regarding silver staining for glycoproteins

Dear All,

           I have this confusion on the silver staining of glycoproteins
IEF gels. I have observed that with increasing glycosylation, the
of the glycoproteins after silver stain decreases. I attribute this to
masking of the aminoacids by the glycan chain resulting in the decrease
efficiency of the silver stain. Has anyone done an IEF for
molecule? Does it give a more intense band than the glycosylated one? My
next experiment would be that but if that is observed by most of  you, I
would not waste my resources on doing that. Kindly give some inputs.

Pradeep Iyer

If you're not part of the solution, you're part of the precipitate -
J. Tillman
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