Content of chondroitin sulfate by USP Method

Dana Summers dsummers at mtcnet.net
Wed Mar 16 10:33:42 EST 2005

I have been following the various changes that have occurred in 
determining the content of chondroitin sulfate by the USP method. Such 
changes have been the the use of a new reference standard (USP 
Chondroitin Sulfate RS), the incorporation of a standard curve USP 27 of 
(0.4mg/mL, 0.8mg/mL and 1.2mg/mL of the standard) to a new standard 
curve in the USP 28 of (0.5mg/mL, 1.0mg/mL and 1.5mg/mL of the standard) 
as well as a change in the buffer from sodium phosphate to now a 
potassium phosphate. The questions I have is can anyone explain the 
benefits of the new changes especially the incorporation of a curve as 
well as a change in the buffer? I have heard from some that  gowing from 
USP 26 which had no curve for the standard to USP 27 that incorporates a 
curve for the standard that their content of chondroitin sulfate in 
their product has dropped ~3%. I would like to hear from all on the 
advantages and disadvantages from all the changes that have occurred 
from the USP testing of chondroitin sulfate.
Dana Summers


More information about the Glycosci mailing list

Send comments to us at biosci-help [At] net.bio.net