Dear Ian,
Here's the method I use.
Preparation of glucose oligomer mixture by partial acid hydrolysate of
dextran with HCl.
To 300mg of freeze-dried dextran (Mr 250,000 from Sigma or equivalent
previously dialysed exhaustively against water) add 3ml 0.1M HCl. Hydrolyse
for 6 hours at 100°C. Cool and pass the hydrolysate through Dowex AG50 and
AG3 resins and filter. Dilute to 50mg/ml. Do a test run on your separation
of choice. The length of the hydrolysis time can be lengthened or shortened
to give the desired level of hydrolysis.
Alternatively, Oxford GlycoSciences sell a "Glucose Homopolymer Standard"
Cat. No. I-4503, 15 mg for 66 pounds.
David Ashford
Ian McFarlane wrote:
>> How does one make a solution of glucose oligomers from Dextran?
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Dr David A. Ashford
Glycobiology: Research & Analytical, Department of Biology,
University of York, P.O. Box 373, York, YO10 5YW UK
Tel: +44 (0)1904 434391 Fax: +44 (0)1904 434312
Email: da5 at york.ac.uk
WWW: http://www.york.ac.uk/depts/biol/staff/daa/daa.htm
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