In article <34995F8A.24D0 at icrf.icnet.uk>, M.Dalziel at ICRF.ICNET.UK (Martin Dalziel) writes:
>Anyone know of a method for de 9-0 acetylation of Neu5,9Ac2 that does
>not effect the c5 acetyl group?
>Is there a specific acetytlase avaliable (i.e. sialate
>9-O-acetylesterase, EC 3.1.1.53)?
>>Martin Dalziel
Hello Martin
Because the 9 acetyl group is a ester group and the 5 is and amide, which is
much more stable to basic hydrolysis. there are quite a lot chemical methods to
differentiate between these two groups. For example aqueous ammonia would only
cleave the 9 acetyl group.
But the best conditions are dependent to your specific problem, are the
Neu5,9Ac2 part of a glycoprotein that will deneutralize under the specic
conditions ...
I hope this hints help you a little.
Christoph Schaub, Universitaet Konstanz
