I agree with Kieran's suggestion to properly control junk postings in our group.
For core alpha 1,6-linked fucose detection, I don't think there is a good lectin
to use. I indirectly detected such a structure by using PNGase F and MALDI-TOF
mass spectrometry. Since PNGase F does not cleave alpha 1,3fucosylated complex
structures, and that treatment of glycopeptide with alpha-fucosidase did not
cause any size changes.
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* Ke-Wei Zhao, Ph.D. Phone: (310)825-8722
* Department of Biological Chemistry Fax: (310)206-5272
* UCLA School of Medicine, CHS 33-257 e-mail: kzhao at biovx1.biology.ucla.edu
* Los Angeles, CA 90095-1737
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