glycoprotein staining

[Kajiwara Hideyuki]

Hi,

 
> I have questions about glycoprotein staining. I have partial purified
> some protein with con-A sepharose. When I electrophoresed the partial

Easiest way to detection of glycoprotein is using Con-A-HRP conjugate
because you use con-A sepharose column.  Con-A-HRP is commercially
available.  If not, it is possible to label by biotin, and detect using
avidin-HRP.

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   Hideyuki Kajiwara

   National Institute of Agrobiological Resources
   Dept. of Biotechnology, Lab. of Gene Engineering
   E-mail: kajiwara at abr.affrc.go.jp
   Tel: 81-298-38-7442 or 7076
   Fax: 81-298-38-7073
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