We are trying to determine if there is sulfated carbohydrate on our
glycoprotein (MW 50000 based on amino acid analysis, 65-70 kDa based on
PAGE). Our favoured technique is HPLC analysis of a trypsin digest of
the protein using on-line electrospray MS (high orifice potential to
break the S-Ocarbohydrate bond). Does anyone know of a complimentary
technique and have a reference for it? Also, does anyone have a good
general reference describing the types of sulfated carbohydrate typically
found in nature? Thank you for all responses.