It seems to me that the research experiment involving the use of galacto-
sylated albumin as a neoglycoconjugate to investigate tumor invasion, had
already been done in a much elegant way by Dr. Kevin G. Rice of Ohio State
University (Tel 614-292-6078; Fax 614-292-7766). As the glycobiology
circle well known that glycoconjugates present on tumor cell surface in
particular are most likely in complex structures, rather than single
sugar modifications. And the carbohydrate binding proteins on the host
cell part, also show remarkable cluster effects for branched oligosacchar-
ide structures vs. single sugar chain. Dr. Rice's work (published in:
J. Biol. Chem. 269: 16195-16202, 1994) revealed beautifully the bio-
distribution of glycoconjugates terminating with Gal, GalNAc in their
respective bi-antennary or tri-antennary complex structures. One major
issue of such glycoconjugate biomimetics, if proved to be useful in
the fighting against tumor invasion, is their extremely high clearance
by the liver, and no one knows what will be the clinical consequences
for such a high concentration in the liver in a long term therapy.
Ke-Wei Zhao, Ph.D.
Dept. of Biol. Chem.
UCLA School of Medicine
Los Angeles, CA 90095-1737
On 21 Jun 1996, Xie Chang wrote:
> Dear Sir:
> I am a university student of Beijing Medical Univ.
> Now I'm doing an experiment about neuglycoconjanguate.
> I have Some Quesions:
> How can I synath a conpound of galactose and album of mice?
> the cost of this method must as cheap and easy as possible.
> Since My lab deos not give me much money for this experiment.
> After that I want to inject this conpound to mice.
> Because as we know ,galactose can supress the immigration of
> tumors,if we connect galactose to a large protein, may be it
> will supress the immigration of tumor better.
> How do you think of my experiment?
> Please give me some advise.
> Thanks a lot!
> Sincerely yours Na Jie