In article (Dans l'article) <32AEF369.6AF9 at ensam.inra.fr>,
urbach at ENSAM.INRA.FR (urbach) wrote (écrivait) :
> Hello all,
>> I try to determine if a protein is glycosylated using SF9 expression
> system.
> I test tunycamicine effect on N-glycosylation.
> To test if "my" protein is o-glysosylated, I would like to use an
> specific inhibitor, like neuraminidase.
> ut is it a good inhibitor?,
> Witch concentration have i to use?
Haem... neuraminidase is not a metabolic inhibitor, it is an enzyme that
hydrolyses the terminal non-reducing neuraminic acid residues (sialic acid
to make it short).
Alternatively, you can use Benzyl N-acetyl-alpha-D-galactosamide, a
competitive inhibitor of N-acetyl-alpha-D-galactosaminyl transferase,
which catalyses the first step in the biosynthesis of most O-linked
glycans.
The product is available through Oxford Glycosystems (distributed by COGER
in France : fax= 01.45.32.71.04)
> Excuse for my question, and my english but i am not a specialist.
There is no problem with that, as long as we can communicate !
> Thank you for answer directly to my e.mail adress
C'est parti ! Avec une reponse en anglais pour le newsgroup, ca peut
toujours servir...
> Serge URBACH
>urbach at ensam.inra.fr> Laboratoire de Biochimie et physiologie moleculaire des plantes
> ENSAM/INRA/CNRS/UNII
> Montpellier
...Olivier
_ _ _
\/ \/
{O} Olivier Nosjean
|_| Laboratoire de Physico-Chime Biologique
||| Univ. C. Bernard - Lyon 1
| France
