>To: glycosci at net.bio.net>From: kzhao at biovx1.biology.ucla.edu>Subject: Better method for deglycosylation...?
>Date: 27 Aug 1996 11:54:33 -0700
>>>The trouble to deglycosylate plant glycoprotein by the conventional
>enzymatic
>digestion (e.g., PNGase F) may be the fact that there is a unique
>Fuc1->3core
>GlcNAc which almost blocks the activity of PNGase F. I dont know if there
>is a 1,3fucosidase that can remove this sugar before you digest with PNGase
>F.
TFMS digestion is likely to work although there may be other effects on the
protein. Boehringer sells a PNGase A which cleaves a1,3-core fucosylated
N-glycans. I don't know if it will cleave the difucosylated core.
Chris West
Department of Anatomy & Cell Biology
University of Florida College of Medicine
Gainesville, Florida 32610-0235 USA
Ph 352-392-3329
Fax 352-392-3305
e-mail westcm at college.med.ufl.edu
