The trouble to deglycosylate plant glycoprotein by the conventional enzymatic
digestion (e.g., PNGase F) may be the fact that there is a unique Fuc1->3core
GlcNAc which almost blocks the activity of PNGase F. I dont know if there
is a 1,3fucosidase that can remove this sugar before you digest with PNGase F.
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* Ke-Wei Zhao, Ph.D. Phone: (310)825-8722 *
* Department of Biological Chemistry Fax: (310)206-5272 *
* UCLA School of Medicine, CHS 33-257 e-mail: kzhao at biovx1.biology.ucla.edu *
* Los Angeles, CA 90095-1737 *
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