Message:
>This graduate student would like to know if there is any advice out there
>on the best filter materials to filter samples containing oligosaccharides,
>glycopeptides, _or_ glycoproteins. I intend to use syringe filtration to
>clean samples of gross particles, minor microbial growth due to extended
>incubation periods (even with NaN3), and such. Probably, no one filter
>type will be suitable for all three applications, but i am overwhelmed by
>the options. I think i've ruled two out so far: nylon and cellulosic
>filters. Oh yes, naturally these will be aqueous solutions, and near
>neutral pH.
>Jeremy
Dear Jeremy,
I believe that kits sold by Oxford GlycoSystems for manipulating glycans use PTFE filters.
Do these work for you?
-Chris West
Chris West
Department of Anatomy & Cell Biology
University of Florida College of Medicine
Gainesville, Florida 32610-0235 USA
Ph 352-392-3329
Fax 352-392-3305e-mail westcm at college,med.ufl.edu
