Frank C Hay (frank at rabbits.demon.co.uk) wrote:
: Further to my query on inhibitors on glycosidase inhibitors is there is a
: simple way of quantitating total oligosaccharide on glycoproteins on
: Western blots?
: I know Oxford Glycosystems has a technique for determining whether a
: protein is glycosylated but they tell me this is not very quantitative.
Maybe a modification of the Boehringer kit?
Boehringer use dig-labelled lectins and an anti-dig antibody linked to
AP. What about dig labelled lectins, and hot anti-dig antibody??
Count by using either a phosphor Imager or cutting out the bands from the
inert support and counting in a gamma counter? Express results as cpm per
ug protein? Use fetuin or some such as a standard and construct a
standard curve.
This is juat an idea, not a solid proposal as yet.
__________________________________________________
Steven Pirie-Shepherd
srps at galactose.mc.duke.edu
"Insert your own pithy phrase just about here!"
