George Weiner (george-weiner at uiowa.edu) wrote:
: We have produced an antibody that we think reacts with an o-linked
: glycosylation variant of HLA-DR. Is there an enzyme or culture
: techniques we can use to remove the o-linked glycosylation of surface
: proteins from intact cells or or lysates?
O-glycosidases are liminted in scope as they only play with Gal->GalNAc as
a substrate, further substitutions such as SA, fucose etc MUST be triommed
with the appropriate exoglycosidases prior to the O-glycosidase (Glyko in
novata CA have a comprehensive list of decent glycosidases). Otherwise it
takes hydrazine to remove all the O-linked sugar on a protein.. I am not
sure what effect tunicamycin has on O-linked glycosylation? Mabe someone
lse has that info?
__________________________________________________
Steven Pirie-Shepherd
srps at galactose.mc.duke.edu
"Insert your own pithy phrase just about here!"
