Hi, Aida:
Electroelution (or any other electrophoretic procedure) should not affect the
detection of glycosylation by the Oxford kit. Electrophoretic procedures are
routinely used in the purification/separation of glycosylated proteins.
John
>On 24-Mar-95 14:13 Aida Cancel {axc19 at psu.edu} wrote:
>>HI! Can anyone tell me if using a protein that has been electroeluted to
>detect possible glycosylation of the protein is accepted for publication?
>In other words: I purified a protein by electroelution and I am
>interested in knowing if it is glycosylated. I bought a glycosylation
>detecting kit (Oxford) but I would like to know if using electroelution
>will harm glycosylation of the protein to the extent that it can not be
>detected.
>>Thanks,
>Aida
