Dear Chisoon Chang:
You wrote that you were worried about amino acids sticking to the
PA-1 column. I don't believe that the amino acids stay on the column.
I know that some amino acids interfere with quantitation of sugars
(i.e. lysine elutes very close to galactosamine). Does any one else
have information about amino acids sticking to the PAC-1 column?
I use a 2N TFA hydrolysis for neutral monosaccharides and 6N HCl
hydrolysis at 100 C for 4 hours for the amino monosaccharides. I am
primarily analyzing glycoproteins. Glycopeptides would probably have
more amino acid interference.
Please contact me if you want more details. My email is cash at bms.com