christer.ericsson at mog.ki.se (Christer Ericsson) wrote:
>Is anything known about N-glycosylated proteins in mitochondria? I am
>worrying that mithochondria might contaminate my cell membrane
>preparation. But since most of my membrane proteins are N-glycosylated
>(since they bind conA) I would imagine that they are unlikely to be
>mitochondrial in origin? I guess I could assay some mitochondrial marker protein but would prefer to spend my time and resources more productively.
>The membrane prep is a standard homogenization/low speed/high speed
>>Appreciate your input.
>Christer Ericsson, Dept. of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, S-171 77 Stockholm, Sweden.
>>CUSeeMe Videoconference 220.127.116.11
A good reference regarding mitochondrial membrane glycoproteins is
Gasnier F et al. Further characterisation of mitochondrial outer membrane: Evidence for the presence of two endogenous sialylated glycoproteins. 1994; J Biochem. 116, 643-648
hope this is of use.
martin at axford.demon.co.uk