Is anything known about N-glycosylated proteins in mitochondria? I am
worrying that mithochondria might contaminate my cell membrane
preparation. But since most of my membrane proteins are N-glycosylated
(since they bind conA) I would imagine that they are unlikely to be
mitochondrial in origin? I guess I could assay some mitochondrial marker
protein but would prefer to spend my time and resources more productively.
The membrane prep is a standard homogenization/low speed/high speed
centrifugation fraction.
Appreciate your input.
Christer
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Christer Ericsson, Dept. of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institute, S-171 77 Stockholm, Sweden.
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