Aida Cancel axc19 at psu.edu
Sat Jan 14 19:22:20 EST 1995

I have been trying to use PAS staining to detect glycoproteins in 10-17.5% 
(1.5 mm) 2D SDS-PAGE gels of seminal plasma proteins...needless to say, 
without success.  I have tried 4 different protocols (including doing the 
staining after transfer of the proteins to nitrocellulose).  My problem is 
that there is little or no color development.  I am loading lots and lots of 
protein...and for a fact I know that many of these are extensively modified 
and have carbohydrates attached.  Can anyone help me?  Any recommendations?

(Please send reply to axc19 at psu.edu)


More information about the Glycosci mailing list

Send comments to us at biosci-help [At] net.bio.net