> Dear GlycoFriends,
> I've seen from the paper(Lodish et al, J.Biol.Chem., 1993, 268, 13639-49)
> that a glycoprotein happen to be Endo H-resistant type, even though it
> has N-linked oligosaccharide. Could this happen because of some
> structural hinderance or something else..?
Only certain N-linked oligosaccharides are susceptible to EndoH and
if the paper is by Lodish I guess it's to do with biosynthesis - so
one can follow the conversion of oligosaccharides from EndoH
sensitive to EndoH resistant as a means of following the transit of a
glycoprotein from the ER to the Golgi due to oligomannose structures
becoming complex (see a brief mention of this in S.R. Carlsson in
Fukuda & Kobata, Glycobiology A Practical Approach, p. 19-20.)
Iain
---------------------------------------------------------------
Iain Wilson Institut fuer Chemie
Tel: 43-1-47654-6065 Universitaet fuer Bodenkultur
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E-mail: wilson at edv1.boku.ac.at A-1180, WIEN, Austria
