Biochemist with five years of postdoctoral experience and publication
record looking for a job in San Francisco Bay area.
Strong background in enzyme (glycosidases and
glycosyltransferases) and membrane receptor (hepatic lectin, a
galactosyl-specific glycoprotein receptor) determination, purification
and characterization.
Solid background in Glycoprotein biosynthesis, processing and
secretion studies.
RIch experience in broad range of biological, biochemical and
immunological techniques, especially in cell and tissue culture
(including normal cells, tumor cells and tissue slices), cell in vivo
radiolabeling, subcellular compartment preparation (including ER, Golgi,
etal), enzymeassays (including radioenzymeassay and spectrophotometric
assay), receptorassays (including radioligand assay and ELISA assay),
immunoassays (including ELISA-enzyme linked immunoabsorbed assay,
radioimmunoassays, competitive bionding), immunoprecipitation,
immunoblot (western blot), liposome preparation, carbohydrate structure
analysis (including TLC, HPLC, chemical and enzymatical methods),
electrophoresis and chromatography (including affinity, gel filtration,
ion exchange, thin layer, high pressure liguid, paper).
Following is my resume. Please feel free to contact me for further
information at
Tel:(408)-296-1327, Email: zhang at ix.netcom.com
Shuai Weng, Ph.D
OBJECTIVE Research Associate position in San Francisco Bay
Area
EXPERIENCE
Research Associate 11/90-04/95
Dept. of Biological Chemistry and Molecular Pharmacology
Harvard Medical School and Joslin Diabetes Center, Boston, MA 02215
Conducted studies in glycoprotein and free soluble
oligosaccharide biosynthesis, processing and subcellular translocation;
glycoprotein processing enzymes (glycosidases) dermination,
purification, characterization, and in vivo function; preparation and
structural analysis of N-linked and free oligosaccharides, et al.
Postdoctoral Research Associate 05/89-10/90
Dept. of Biochemistry and Chemistry
University of Notre Dame, Notre Dame , IN 46556
Conducted studies in the purification and characterization of
membrane bound glycosyltransferases involved in glycolipid biosynthesis
by affinity chromatography
Research Assistant/Ph.D. student 09/83-10/88
Dept. of Biochemistry, Shanghai Medical University, Shanghai, China
Conducted studies in determination, purification and
characterization of hepatic lectin (membrane receptor of glycoprotein)
and dynamic study during carcinogenesis.
EDUCATION
Ph.D. in Biochemistry, Shanghai Medical University, Shanghai,PRC 1988
B.S. in Pharmaceutical Chemistry, Shanghai Medical University 1983
PUBLICATIONS
Weng,S., and Spiro, R.G.:Demonstration that a kifunensine-resistant
mannosidase with a unique processing action on N-linked oligosaccharides
occurs in rat liver endoplasmic reticulum and various cultured cells. J.
Biol. Chem. 268: 25656-25663 (1993)
Weng,S., and Spiro, R.G.: Endoplasmic reticulum kifunensine-resistant
mannosidase (ER mannosidase II ) is enzymatically and immunologically
related to the cytosolic mannosidase. J. Biol. Chem. (in press)
Weng,S., and Spiro, R.G.: Another three papers about glycoproteins and
their processing enzymes are under preparation
Basu,M., Weng,S., Das, K.K., and Zhang, B.: Regulation of galactosyl-
and fucosyltransferase of tumor cell origin. (Abstr.) Glycoconjugate J.
6: 372 (1989)
Chen, R., Weng, S., Zhang, D., Zhang, X., and Gu, T.: Targeting of
galactosyl-containing liposomes to liver. Acta Biochim. Biophys. Sinica
20: 312-316(1988)
Weng,S., Chen, Q., and Gu, T.: Studies on the decrease in activity of
hepatic lectin in liver cancer. Acta Biochim. Biophys. Sinica 19,
152-156 (1987)
Weng, S., Chen, Q., and Gu, T.: Loss of hepatic lectin in hepatoma.
Tumor 7: 10-13 (1987)
Weng,S., Chen, Q., and Gu, T.: Studies of dynamic changes of hepatic
lectin activity during carcinogenesis. Chinese Biochem. J. 3: 93-95
(1987)
