I am trying to standardize a PCR method for Bcl1 rearrangement for
mantle cell lymphoma. I am using primers used by several groups and
published but am unable to reproduce results. I have done the usual
Mg++ concentration titration and different annealing temperatures.
Is there anyone who has had to standardize this particular
rearrangement technique who can give me some hints or is willing to
dialogue with me. I need help!