[This listserv seems to impose a fairly short line-wrap for
text messages, which made my previous post difficult to
read. Hence this re-send, with shorter line lengths, where
possible.]
Greetings GenBank Users,
The PROJECT linetype allows a sequence record to be linked to
information about the sequencing project that generated the data
which ultimately resulted in the record's submission to the
International Nucleotide Sequence Database ( INSD; see
http://www.insdc.org ).
This complete bacterial GenBank record illustrates the use of
the PROJECT line:
LOCUS CP000964 5641239 bp DNA circular BCT
24-SEP-2008
DEFINITION Klebsiella pneumoniae 342, complete genome.
ACCESSION CP000964
VERSION CP000964.1 GI:206564770
PROJECT GenomeProject:28471
When viewed on the web in NCBI's Entrez:Nucleotide, the record's
project identifier (28471) links to an entry in the Genome Project
Database (GPDB) :
http://www.ncbi.nlm.nih.gov/sites/entrez?db=genomeprj&cmd=Retrieve&dopt=
Overview&uid=28471
where information about the sequencing center, the bacterium, and
other GenBank records (eg, plasmids) associated with the sequencing
project can be found.
Since the introduction of PROJECT, the scope of the "Genome" Project
Database has expanded, to include projects that are not necessarily
targetted to the sequencing of a complete genome.
In addition, there can be other resources which underlie an INSD
sequence record, such as the Trace Assembly Archive at the NCBI:
http://www.ncbi.nlm.nih.gov/Traces/assembly/assmbrowser.cgi?cmd=show&f=t
ree&m=main&s=tree
Because of the expanded scope of the GPDB, and because we
anticipate a need to link to more resources than just the GPDB,
the PROJECT linetype is going to be replaced by a new linetype:
DBLINK
Further details about this change, and its timetable, follow.
Mark Cavanaugh
GenBank
NCBI/NLM/NIH/HHS
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Modifications to linetypes can be disruptive, so the switch to
DBLINK will occur in several stages.
Starting in October 2008, links to the NCBI Trace Assembly Archive
will be supported via a line of text in the COMMENT section of
sequence records.
Here is a mock-up, based on CP000964, to illustrate this change:
LOCUS CP000964 5641239 bp DNA circular BCT
24-SEP-2008
DEFINITION Klebsiella pneumoniae 342, complete genome.
ACCESSION CP000964
VERSION CP000964.1 GI:206564770
PROJECT GenomeProject:28471
....
COMMENT Trace Assembly Archive:123456
The source for the DNA and/or cells is: Professor Eric W.
Triplett, Chair, Department of Microbiology and Cell
Science,
Institute of Food and Agricultural Sciences, University of
Florida,
P.O. Box 110700, Gainesville, FL 32611-0700, ewt from ufl.edu.
Note: Use of the Trace Assembly Archive is still in its early
stages, so only a few records are expected to have these links in
the short term.
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The new DBLINK linetype will be introduced as of GenBank Release
170.0 (February 15, 2009) .
The Genome Project ID and the Trace Assembly Archive ID will be
presented via DBLINK, and the existing PROJECT line will continue
to be displayed:
LOCUS CP000964 5641239 bp DNA circular BCT
24-SEP-2008
DEFINITION Klebsiella pneumoniae 342, complete genome.
ACCESSION CP000964
VERSION CP000964.1 GI:206564770
PROJECT GenomeProject:28471
DBLINK Project:28471
Trace Assembly Archive:123456
....
COMMENT The source for the DNA and/or cells is: Professor Eric W.
Triplett, Chair, Department of Microbiology and Cell
Science,
Institute of Food and Agricultural Sciences, University of
Florida,
P.O. Box 110700, Gainesville, FL 32611-0700, ewt from ufl.edu.
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PROJECT and DBLINK will co-exist for one GenBank release, until
Release 171.0 (April 15, 2009), at which point the PROJECT line
will be removed.
In its final state, our mock-up for CP000964 becomes:
LOCUS CP000964 5641239 bp DNA circular BCT
24-SEP-2008
DEFINITION Klebsiella pneumoniae 342, complete genome.
ACCESSION CP000964
VERSION CP000964.1 GI:206564770
DBLINK Project:28471
Trace Assembly Archive:123456
....
COMMENT The source for the DNA and/or cells is: Professor Eric W.
Triplett, Chair, Department of Microbiology and Cell
Science,
Institute of Food and Agricultural Sciences, University of
Florida,
P.O. Box 110700, Gainesville, FL 32611-0700, ewt from ufl.edu.
In summary:
PROJECT -> DBLINK
'GenomeProject' -> 'Project'
Additional linkages, such as Trace Assembly, added to DBLINK
as-needed
The PROJECT line will be removed as of April 15 2009.
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For those who process sequence data in NCBI's ASN.1 format:
The underlying representation for (Genome) Project IDs will remain
unchanged; there will be no changes to the ASN.1 User-object that
is used to store them:
user {
type
str "GenomeProjectsDB" ,
data {
{
label
str "ProjectID" ,
data
int 28471 } ,
{
label
str "ParentID" ,
data
int 0 } } } ,
However, to support linkages to other resources, like the Trace
Assembly Archive, a new "DBLink" User-object will be introduced:
user {
type
str "DBLink" ,
data {
{
label
str "Trace Assembly Archive" ,
data
ints { 123456 } } } }
As new types of linkages are established, they will be added to
the DBLink User-object, and displayed via the DBLINK linetype in
the GenBank flatfile format.
There is a possibility that the GenomeProjectsDB User-object
might someday be incorporated into the new DBLink User-object.
But at the moment, there are no firm plans to do so.
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