dsred2 / eGFP in vivo

Hannes Klump klump.hannes at mh-hannover.de
Thu Oct 2 07:28:18 EST 2003

Hi out there!

I have two questions for you regarding the use of dsRed2 (and GFP) in
primary cells:
first: did any of you experience any toxicity or strange effects of dsRED2
on such cells yet? I have the feeling that this protein does not behave
"neutral" in hematopoietic cells, but leads to strange selective effects.
2.) when coexpressing GFP and dsRed2 - do you have any problems with
compensation during FACS analysis? We are using a FACScalbur from BD and at
least in my hands, dsRED2 (measured in FL2) heavily emits into the FITC
channel (FL1).

Any ideas?

Hannes Klump, Ph.D.
Hannover Medical School
Center of Internal Medicine
Dept. of Hematology and Oncology
Experimental Cell Therapy, OE5120
Carl-Neuberg-Strasse 1
30625 Hannover
Tel. (++49)511 532 9473
Fax.: (++49)511 532 4521
e-mail: klump.hannes at mh-hannover.de


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