Hi out there!
I have two questions for you regarding the use of dsRed2 (and GFP) in
first: did any of you experience any toxicity or strange effects of dsRED2
on such cells yet? I have the feeling that this protein does not behave
"neutral" in hematopoietic cells, but leads to strange selective effects.
2.) when coexpressing GFP and dsRed2 - do you have any problems with
compensation during FACS analysis? We are using a FACScalbur from BD and at
least in my hands, dsRED2 (measured in FL2) heavily emits into the FITC
Hannes Klump, Ph.D.
Hannover Medical School
Center of Internal Medicine
Dept. of Hematology and Oncology
Experimental Cell Therapy, OE5120
Tel. (++49)511 532 9473
Fax.: (++49)511 532 4521
e-mail: klump.hannes at mh-hannover.de