Hi out there!
I have two questions for you regarding the use of dsRed2 (and GFP) in
primary cells:
first: did any of you experience any toxicity or strange effects of dsRED2
on such cells yet? I have the feeling that this protein does not behave
"neutral" in hematopoietic cells, but leads to strange selective effects.
2.) when coexpressing GFP and dsRed2 - do you have any problems with
compensation during FACS analysis? We are using a FACScalbur from BD and at
least in my hands, dsRED2 (measured in FL2) heavily emits into the FITC
channel (FL1).
Any ideas?
Cheers,
Hannes
--
Hannes Klump, Ph.D.
Hannover Medical School
Center of Internal Medicine
Dept. of Hematology and Oncology
Experimental Cell Therapy, OE5120
Carl-Neuberg-Strasse 1
30625 Hannover
Germany
Tel. (++49)511 532 9473
Fax.: (++49)511 532 4521
e-mail: klump.hannes at mh-hannover.de
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