We have recently published a paper doing this:
Yu Chen and Sanford M. Simon
In Situ Biochemical Demonstration that P-Glycoprotein Is a Drug Efflux Pump
with Broad Specificity
J. Cell Biol. 2000 148: 863-870.
If you have any questions, please feel free to contact us.
>Subject: Enzyme kinetics
>Date: 6 May 2000 17:26:57 +0100
>>I would like to determine the kinetics of an enzyme in living cells
>using mutant GFPs as the reporter. I would like to monitor the catalytic
>activity of the enzyme. I am very much interested in being able to
>extract quantitative data about the enzymes activity.
>>Does anyone know of any research along these lines using GFPs?
>>Any help would be most helpful.
>urbane45 at netscape.net>>---
Laboratory of Cellular Biophysics
1230 York Avenue
New York, N.Y. 10021
(212) 327-8130 (voice)
(212) 327-8022 (fax)
simon at rockvax.rockefeller.edu (e-mail)
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