Hi,
A question to all of you using DsRed.
I recently measured the excitation-/emmision spectra if a
DsRed-fusionprotein expressed in HEK293T cells. I found a very narrow
excitation spectrum with a peak at 572 nm, ranging from app. 560 to 590 nm,
instead if the broad excitationspectrum described by Clontech (max.: 558
nm). The emmisionspectrum of the fusion protein had a very broad peak (from
app. 595 to 615 nm) with 50% max. values at 585/635 nm, significantly
different to the one published (max.: 583 nm).
a) has anyone seen similar spectra for their fusionproteins ?
is this a general phenomenon ?
b) if so, this might be a reason why some dsRed-constructs can not be
excitated by a standard argon laser (488/514 nm) as a light source, as some
people have complained in here.
Waiting for your comments,
(with burning patience),
Holger Fehr
---------------------------------------------------
Dr. Holger Fehr
Inst. Physiological Chemistry II
University of Wuerzburg
Germany
fehr at biozentrum.uni-wuerzburg.de
---
