HeIlo, I'am new in the eGFP field, and I will ask the following
Does any one known what will be the minimal deletion in the 3' end of
the eGFP coding sequence to knock-out the transcription and/or the
traduction and/or the fluorescence of the eGFP, in mammalian cell
transfected with the peGFP-C3 vector ( without deleting the three STOPs
codon). What happen if we remove the three last amino-acid of the eGFP ?
Thank, I' m waiting for replies.