SAINTIGNY Yannick 161011 wrote:
> Does any one known what will be the minimal deletion in the 3' end of
>the eGFP coding sequence to knock-out the transcription and/or the
>traduction and/or the fluorescence of the eGFP, in mammalian cell
>transfected with the peGFP-C3 vector ( without deleting the three STOPs
>codon). What happen if we remove the three last amino-acid of the eGFP ?
As far as I remember (Roger Tsien mentioned it at the last ASCB meeting),
one can delete a maximum of about 5 AA on the c-terminus of GFP and still
get a fluorescent protein. The situation should be the same for eGFP.
Beat
-----------------------------------------------------
Dr. Beat Ludin, FMI, PO Box 2543, 4002 Basel, Switzerland
Tel. +41 61 697 6697 / FAX +41 61 697 3976
Internet:ludin at fmi.ch / Compuserve:100102,1527
