GFP in HeLa cells

Joe Binder jjbinder at students.wisc.edu
Wed Apr 30 16:52:08 EST 1997

You were right.  I do have the double mutant.  Unfortunately, I don't 
have much information about it and attempts to find out more haven't 
been answered.  Here's what I do know:

In C.elegans I believe, the double mutant appeared to be brighter than 
the original GFP or the ind. mutants alone, when driven under the 
unc-25 and vab-3 promoters.

They have broader emission and excitation spectra (rhodoamine filters 
still show flourescence, although not as bright as fluor.).

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