GFP and Flow Cytometry

[Simon D. Scott]

Hello. We're investigating the use of wild-type and mutant GFPs in live 
and fixed cells (e.g. MCF7 breast tumour line) and wish to quantitate 
using FLOW CYTOMETRY. We are counterstaining fixed cells with PI. 70% 
ethanol is the fixative.
 Any advice? - Simon Scott/Brian Marples PICR