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intron in transgenic mice

Beat Ludin ludin at FMI.CH
Wed Apr 16 01:59:03 EST 1997

SAINTIGNY Yannick 161011 wrote:

>Hello, in a transgenic mice, is it absolutely necessary to insert a
>intron in the plsamid construction withe EGFP ?

AFIK, current thinking says that an intron is usually required for 
efficient nuclear export of any mRNA. You will get much lower expression 
levels without, but I don't think that it as absolutely necessary

Dr. Beat Ludin, FMI, PO Box 2543, 4002 Basel, Switzerland
Tel. +41 61 697 6697 / FAX +41 61 697 3976
Internet:ludin at fmi.ch / Compuserve:100102,1527

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