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Expression of GFP in Bacillus subtilis

Dr. George McNamara george_m at image1.com
Mon Apr 7 14:59:10 EST 1997

Dear Dr. Xu,

  C.D. Webb et al (1997) Cell 88:667-674 and (1995) J. Bacteriol.

  The 1997 paper is in the March 7 issue and uses the very slick GFP-LacI
binding to tandem repeats of the Lac operator. See also Straight et al
(1996) Current Biology 6:1599-1608 and Robinett et al (1996) J. Cell Biol.
135:1685-1700 for use in other cell types and applications.


p.s. You could have found the Webb et al (1995) paper with a Medline title
search of "green" and "subtilis".

>Has anyone expression the GFP in Bacillus subtilis? I hope it could be
>expressed in B. subtilis without any inducer and it will be stable for
>wild B. subtilus strain. 
>Jianping Xu
>Associate Frofessor
>Biotechnology Institute
>Zhejiang Agricultural University
>268# Kaixuan Road
>Hangzhou 310029
>E-mail: xujp at hotmail.com

George McNamara, Ph.D.
Applications Scientist
Universal Imaging Corporation
502 Brandywine Parkway
West Chester, PA 19380 USA
voice:	610-344-9410 ext 224
fax:	610-344-9515
internet:	www.image1.com

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