Historically and typically, by observing distinct cytopathic effect
human fibroblast cells in tissue culture tubes. This is slow and may
to 4 weeks to display the CPE. Our hospital also utilizes a "shell
FITC-conjugated monoclonal antibody staining procedure. In this
patient's specimen is added to a shell vial that has a round coverslip
bottom of the vial. The coverslip is layered with human fibroblasts.
patient's specimen is added to a set of shell vials and the shell vials
centrifuged for about 1 hour. Culture media is added to the shell vial
the shell vial is incubated at 37 degrees. At different time points
course of 1-7 days post inoculation, a shell vial is removed from the
incubator and stained with a FITC-conjugated monoclonal antibody
against a CMV early nuclear antigen. The stained round coverslips are
on a slide and perused under a UV scope for cells that show distinct
nuclear staining (almost like "glowing kidney-shaped" nuclei).
we have used for both of these procedures include human white blood
harvested from a buffy coat separation, urine, and inguinal swabs
viral transport medium.
A "positive" IgM antibody test run on serum indicates an active or
I've been a few years away from this, but I'm told that some kits have
developed that utilize PCR to detect CMV (these are Roche kits). I
that other companies have probably developed various configurations of
and indirect fluorescent microscopy for the detection of CMV antigen.
daemon at hgmp.mrc.ac.uk, [mailto:daemon at hgmp.mrc.ac.uk], On, Behalf, Of
> How is CMV diagnosis usually done? Specifically, which lab tests can
>> Paula Lavery
> Transplant Immunology Lab.
> Royal Victoria Hospital
> McGill University
> Montreal (QC) Canada
>> "If we knew what we were doing it wouldn't be called research, would
> -- Albert Einstein