I have a problem in conjugating a monoclonal antibody to 20 nm gold
particles. Monoclonal pi is close to 6.5 and several trials have been
performed at ph 7, 8 and even 9 without significant increase in the
conjugate sensitivity. In our procedure 0.01 mg IgG are incubated with
1 ml of gold particules (at 0.01 %).
I am looking for a protocol which enables me to increase this conjugate
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