On 16 Mar 1995, Robert Burns wrote:
> Hi all,
> my first posting to the "now permanent" diagnost list
>> My question: Has anybody raised Mabs using protein bands excised
> from PAGE gels as the immunogen? If so can you use the gel band
> itself as an implant without having to elute the protein?
>> Second question ( I have asked this before but for the benefit of
> newcomers) Is anybody interested in Fungal diagnostics by serological
> methods?
>>> Robert
>>>>>> Robert Burns
>> Monoclonal Antibody Unit
> Scottish Agricultural Science Agency
> East Craigs
> Edinburgh
> Scotland
>>burns at sasa.gov.uk>>>>Yes, you could immunize with the gel without elution, but macerate
(grind) it first to smaller particles.
Falk Fish.
