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Mabs from PAGE bands

Falk Fish ila2027 at ZEUS.DATASRV.CO.IL
Mon Mar 20 23:20:12 EST 1995




On 16 Mar 1995, Robert Burns wrote:

> Hi all,
>      my first posting to the "now permanent" diagnost list
> 
> My question: Has anybody raised Mabs using protein bands excised
> from PAGE gels as the immunogen? If so can you use the gel band
> itself as an implant without having to elute the protein?
> 
> Second question ( I have asked this before but for the benefit of
> newcomers) Is anybody interested in Fungal diagnostics by serological
> methods?
> 
> 
> Robert
> 
> 
> 
> 
> 
> Robert Burns
> 
> Monoclonal Antibody Unit
> Scottish Agricultural Science Agency
> East Craigs
> Edinburgh
> Scotland
> 
> burns at sasa.gov.uk
> 
> 
> 
> 
Yes, you could immunize with the gel without elution, but macerate 
(grind) it first to smaller particles.

Falk Fish.




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