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Immunohisto marker for macrophages needed

CHELACK at ADMIN3.USASK.CA CHELACK at ADMIN3.USASK.CA
Wed Mar 15 09:47:57 EST 1995


Hi, I keep getting requests for a staining paraffin sections for macrophages
in particular cases of suspected histiocytic tumors in dogs.  What we are 
looking for is a marker for macrophages/histiocytes in paraffin sections 
which could be used in any species.  Is there anyone out there in netland
who is already doing this?  My initial thoughts are that an antibody to 
myeloperoxidase or alpha 1 anti tryosin might be the way to go but I am 
short on time and wonder if anyone has validated such an antibody on paraffin
sections, or for that matter in other species.  Most of the commercially
available antibodies such as Mac-1 or Mac-3 do not seem to work on paraffin
sections of canine tissues so I am looking for a more generic marker.  
Thanks for any advise.

			Regards
			BJC - Brian	Short people... last to know its
					raining, first to know its a flood.



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