I hope that this is the appropriate newsgroup for these questions... I am attempting the preparation of a karyotype of the amphipod,Corophium volutator using a standard protocol that employs colchicine and heated 10% Giemsa stain.
I would be grateful for any methods/tips that anyone has found useful,with marine crustaceans especially. Specifically, I am wondering about standard times for the staining process. My literature review has turned up several staining times ranging from 20 min. to 3 h.
Also, I am curious whether the appearance (colour) of the stained squash varies with staining time.
If you can help me with these questions, I would appreciate it if you would email responses to: 901106c at dragon.acadiau.ca.