The primer sequences which we found give somewhat more accurate
sequence reads are, for the M13 Forward primer direction :
aaacgacggccagtgag
and for the M13 Reverse primer direction :
caattaaccctcactaa
I would be happy to hear of results (positive, negative, or
neutral !) from anyone who compares these to other sequencing
primers.
Greg Lennon
Human Genome Center
Lawrence Livermore National Laboratory
> From BIOSCI-REQUEST at net.bio.net Thu Dec 29 06:45:13 1994
> To: biochrom at net.bio.net> From: <cain at icr.ac.uk>
> Subject: pBluescript primers
>> Dear Newsreaders ,
>> I am looking for either the referance or sequence of the
> improaved Bluescript primers mentioned in the paper by J Perry and
> G Lennon FASEB Journal 94 vol 8 no 7 pg A1362 . They are said to
> prime moreaccuratly than the standard commercial primers thus
> giving better reads .
> I will make sure that the sequence is posted on the
> newsgroup if anyone can supply it along with any follow up results
> I have .
>> Sincerely ,
>> David Cain
> Email cain at icr.ac.uk>>
