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reverse patch-clamp

Stefan=Schuster at rz.hu-berlin.de Stefan=Schuster at rz.hu-berlin.de
Thu May 19 03:54:55 EST 1994


Re: Reverse patch-clamp
  
Edwin Donath, who works in our institute of Biophysics and is a specialist in 
patch-clamp, asked me to forward the following message to the net. His e-mail 
address is Edwin=Donath @ rz.hu-berlin.de. 

This procedure is largely in use in patch clamping in the whole cell mode. 
But one should consider that in parallel many substances will leak out of the 
cell into the pipette. It is also unclear how to see any other effects except 
electrophysiological changes because a whole cell patch is irreversible.

Stefan Schuster




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