Hello,
In my opinion your database is not in blastable format. You have to use
formatdb command:
http://goo.gl/Vy114
I hope will be useful,
Victoria Dominguez
ABgenomica
On Tue, Jul 5, 2011 at 11:08 AM, ishabioinfo <srivastavaisha.06 from gmail.com>wrote:
> hii
>> i have downloaded the latest version of local blast ncbi-
> blast-2.2.25+.
> created .ncbirc file .
> downloaded nr database.
> when i m giving the command -
>> blastall -p blastp -i mixo2.fasta -d nr -o fasta.out
>> it is showing the error ---
>> "Could not calculate ungapped Karlin-Altschul parameters due to an
> invalid query sequence or its translation. Please verify the query
> sequence(s) and/or filtering options"
>> kindly help me to solve this problem
>> thanku :)
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