GCG has the best primer selection tool, in my experience.
"Helene Chable" <helene.chable at unilim.fr> wrote in message
news:220.127.116.11.20040115130808.003c5980 at pop.unilim.fr...
> does someone know an efficient software for primer design? We perform PCR
> with the LightCycler instrument and it is difficult to amplify highly
> diluated DNA because primer dimer make competition with target
> amplification (whereas primer are very efficient for highly concentrated
> DNA that is well amplified)
> Does someone have a solution?