Primer3. Two thumbs up. Simple, yet lots of features if you want them.
Charlie
On 12 Jun 2002, Chris Upton wrote:
>> Hi,
> I'm looking for a simple PCR primer design program.
>> I want to amplify a series of genes, so my primers are pretty much
> fixed in position.
> I want to calculate Tm and look for potential hairpins and primer
> dimers from my pairs of primers.
> But I don't want to have to enter the target sequence for all of these.
>> Any suggestions?
>> Thanks,
> Chris
>>
