Gel image analysis questions

philipp.pagel at cmp.yale.edu philipp.pagel at cmp.yale.edu
Mon Feb 25 09:32:57 EST 2002

maneesh <maneesh at drunkenbastards.com> wrote:

> Quantitation:
> Are most gel photographs 8bit deep images form CCD?  I've often found
> people
> guestimate amounts of DNA from these images, but when you view them at
> different exposures, you can get wildly different answers.
> I'm considering implementing a method for recovering a "linear"
> response map
> from a set of differently exposed images for more accurate
> quanitation; but before I do I was wondering if the non-linearity of
> the camera response has ever been examined in detail with respect to
> quantitation from gels...

One very important thing to keep in mind is also that even if you get
the scanner/camera response in the linear range your signal on the film
might not respond in a linear fashion at all. When quantitating e.g.
Western-Blots one should include a serial dilution of standards to take
care of this.


Dr. Philipp Pagel
Department of Cellular and Molecular Physiology        phone: (203) 785-6835	
Yale University                                        fax:   (203) 785-4951
New Haven, CT 06520, USA

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