maneesh <maneesh at drunkenbastards.com> wrote:
> Are most gel photographs 8bit deep images form CCD? I've often found
> guestimate amounts of DNA from these images, but when you view them at
> different exposures, you can get wildly different answers.
> I'm considering implementing a method for recovering a "linear"
> response map
> from a set of differently exposed images for more accurate
> quanitation; but before I do I was wondering if the non-linearity of
> the camera response has ever been examined in detail with respect to
> quantitation from gels...
One very important thing to keep in mind is also that even if you get
the scanner/camera response in the linear range your signal on the film
might not respond in a linear fashion at all. When quantitating e.g.
Western-Blots one should include a serial dilution of standards to take
care of this.
Dr. Philipp Pagel
Department of Cellular and Molecular Physiology phone: (203) 785-6835
Yale University fax: (203) 785-4951
New Haven, CT 06520, USA