IUBio

Free Online Plasmid Map Service

Harry Mangalam mangalam at home.com
Fri Oct 19 10:54:47 EST 2001


Hi All,

tacg is a commandline pattern matching app for nucleic acids that runs on
*nix OSs (as well as on win32 with some cygwin/ming32 coercion).  It's full
range of features are described at:
http://24.1.123.214/tacg3.

However, I had a few days to code again and I whipped up an extension to tacg
that allows it to produce plasmid maps from the info that's stored internally
and usually written as tables.  

You can try it out at:
http://24.1.123.214/tacg3/formt.html

It produces postscript and pdf maps like this:
http://24.1.123.214/tacg3/degenMap.pdf

in response to uploaded sequence (up to 500K - bandwidth restriction, not a
programmatic one) in most formats.  It also produces nested ORF maps in any/all
frames and also notes degeneracies on the outer rim as dark areas.

It would be useful for those labs not having access to a commercial plasmid map
drawing program or for those who need very large plasmids drawn.  Requires
nothing to be downloaded, just a web browser and sequence.  You can also upload
your own set of restriction enzymes or patterns to be searched.

This is alpha code so I haven't added it to the base tacg code yet.  after I'm
satisfied that it's stable, I'll roll it into the std package.

Suggestions as to performance, aesthetics, features, etc welcomed.


-- 
Cheers, Harry

Harry J Mangalam -- (949) 856 2847 (v&f) -- mangalam at home.com 
                    [plain text appreciated]





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