I'm doing a survey on the importance of various features in
2D-gel image analysis software. Could you rate the following
features?
1 ----- 2 ----- 3 ----- 4 ----- 5
unimporant very important
============== 2D-Gel Software-Feature Survey ============
1. Image Cleanup options
(eg, smoothing, background removal, contrast enhancement
etc.)
SCORE = ?
2. Spot Detection:
2a. Availability of adjustable parameters
(eg, min. spot area/size, partial threshold,
confidence etc..)
SCORE = ?
2b. Availability of spot editing options
(eg, adding, deleting, spliting, merging of spots
etc..)
SCORE = ?
3. Gel Alignment:
3a. Speed of alignment:
(consider automatic alignment vs landmarking
technique etc..)
SCORE = ?
3b. Easy visualization of alignment
(dual channel [two colour] display of gels etc.)
SCORE = ?
4. Gel matching:
(consider matching speed, easy visualization of
qualitative/quantitative differences using different
colours])
SCORE = ?
5. Composite Gel:
5a. Adjustable parameters:
(eg, number of gels which should have a spot for it
to be entered in composite gel etc., also consider
appearance of the composite gel)
SCORE = ?
6. Reports:
(consider options available for displaying various
information, export capabilities, etc.)
SCORE = ?
7. Quantitation:
(consider calibration options, histogram for protein
expression differences etc..)
SCORE = ?
8. Statistical Analysis:
(Availability of various statistical methods for
data Analysis)
SCORE = ?
9. Database Querying Option:
SCORE = ?
---------------------------------------------------------------------
General questions:
1. Which step do you think is the current bottleneck in
Proteomic gel analysis?
2. What is/are the software you have experience with and in
your opinion, what is one aspect of your software which
needs improvement?
Regards
Babu
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