IUBio

DB searching with short peptides from phage display

William R. Pearson wrp at alpha0.bioch.virginia.edu
Mon Aug 13 04:22:19 EST 2001


For short peptides, you need to be certain to use a "shallow" scoring matrix.

FASTA with ktup=1 and "-s M10" or "-s M20" can reliably identify sequences
that are 70% - 85% identical given 10 - 15 residues of sequence.

Bill Pearson





More information about the Bio-soft mailing list

Send comments to us at biosci-help [At] net.bio.net