I would like to ask if there is any software for restriction
mapping on an unknown sequence. The programs I found so far
only map the restriction sites on a known input sequence. If I
have a DNA fragment whose sequence is unknown and cut it with
various enzymes, I will see bands on a piece of gel. Is there
any program that will generate a restriction map for me
if I input the sizes of various bands from single and double
digestions?
Thanks a lot!