>I would like to ask if there is any software for restriction
>mapping on an unknown sequence. The programs I found so far
>only map the restriction sites on a known input sequence. If I
>have a DNA fragment whose sequence is unknown and cut it with
>various enzymes, I will see bands on a piece of gel. Is there
>any program that will generate a restriction map for me
>if I input the sizes of various bands from single and double
>digestions?
Have you ever tried COMAP?
Here is the description from the Biocatalog:
AC BC00201
NAME COMAP
DOMAIN Restriction maps
DESCRIPTION programm for helping with the construction of
DESCRIPTION restriction maps of small DNA fragment from digestion
DESCRIPTION data. The program works under a graphical user
DESCRIPTION interface, similar to the one used with the protein
DESCRIPTION analysis program PROFILEGRAPH.
AUTHOR Hofmann K
AUTHOR khofmann at cipvax.biolan.uni-koeln.de
RT -
ADDRESS Universitaet Koeln , Germany
CONTACT -
SITE ftp anonymous src.doc.ic.ac.uk
SITE Directory /usenet/comp.archives/auto/bionet.software
This is a graphical DOS-program, which allows you to mark the bands on
a scanned image of your gel and helps you with construction of the
map. Has an unusal interface and AFAIK there were no updates the last
years.
downloadable from here, e.g., as "comap.zip":
http://microbiol.org/files/biochem.htm
good luck
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Werner Wolz, PhD
Institute of Human Genetics
Biocenter
Wuerzburg/Germany
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