>I'm trying to find a computer program, preferably Win32 compatible but possibly
>Linux compatible that will identify optimal primer sequences for PCR based on a
>multiple sequence alignment. i.e. If I align a set of sequences from a single
>genus that have some variation, I would like to find the best primers to amplify
>all members of the set. Any ideas would be most appreciated.
Hi,
I wrote a DOS program to facilitate this kind of work. You must first
do a multi-alignment with CLUSTALW and then open the .ALN file with my
program. This program is called PRIMERS and it is free, try it at:
http://annhyb.free.fr/others_programs.html
Bye
Olivier
P.S. Actually I am working on the graphic version but the project is
not very avanced
